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. Author manuscript; available in PMC: 2018 Oct 16.
Published in final edited form as: Methods Enzymol. 2016 Feb 1;573:345–362. doi: 10.1016/bs.mie.2015.12.005

Fig. 2.

Fig. 2

FPLC purification of a methyllysine reader domain. (A) Chromatogram from Source 15Q purification. The UV trace at 280 nm is shown in black and the percentage of 1 M KCl buffer is shown in gray. SDS-PAGE analysis of the major peak is shown in the inset. (B) Chromatogram from the subsequent Superdex 75 purification. The UV trace at 280 nm is shown and the SDS-PAGE analysis of the major peak is shown in the inset.