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Troubleshooting
| Problem | Potential Cause and Solution |
|---|---|
| Protein is detected in the negative control | The GST-tagged domain is sticking to the resin. Try increasing the salt concentration of the washes, Triton X-100 concentration and/or the number of washes |
| Signal is very weak as compared to positive control | The wash conditions may be too stringent. Try lowering the salt concentration or Triton X-100 concentration in the wash buffer |
