Figure 5.

DPN or Tam treatment increases QR and ERβ levels and decreases ERα levels. A–C) Western blot analyses of QR (A), ERβ (B), and ERα (C) expression in mammary glands from placebo-, DPN-, or Tam-treated Arom mice. Blots were probed with cytokeratin 18 as a loading control. Expression in the mammary glands of placebo treated mice was given an arbitrary value of 1, and expression in the DPN- or Tam-treated mice was plotted relative to the placebo. Error bars represent means ± 2 SE from ≥4 mice/treatment. D) Representative Western blots of QR, ERβ, ERα, and cytokeratin 18 levels in mammary glands of Arom mice treated with placebo, DPN, or Tam.