Skip to main content
. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: J Med Chem. 2018 Aug 31;61(17):8029–8047. doi: 10.1021/acs.jmedchem.8b01133

Figure 4:

Figure 4:

The effects of non-electrophilic activators 17 (orange) and 23 (purple) and positive control CDDO-Im on the nuclear accumulation of NRF2 in HaCaT Cells. (A) HaCaT cells were treated with DMSO (vehicle) or indicated compound (10 μM) for 6 hours and 24 hours, Nuclear (N) and cytosolic (C) fractions were prepared, equal amount of protein was immunoblotted using antibodies specific for NRF2. β-actin / Lamin A/C was used as loading control. (B) Quantification of Nrf2 band intensity using β-actin as reference. DMSO-treated sample value was used as one arbitrary unit. Error bars represent the standard error from four independent experiments. *:p<0.05; **:p<0.01; ***:p<0.001; if no * is indicated, p>0.05.