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. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: J Med Chem. 2018 Aug 31;61(17):8029–8047. doi: 10.1021/acs.jmedchem.8b01133

Figure 5:

Figure 5:

The effects of non-electrophilic activators 17 (orange) and 23 (purple) on NRF2 target gene expression in HaCaT Cells. (A) HaCaT cells were treated with DMSO (vehicle) or indicated compound (10 μM) for 6 hours and 24 hours. cDNA were prepared from mRNA and analyzed for the target gene expression (A: HMOX1; B: NQO1; C: GCLC; D: GCLM; E: SOD) using qPCR with specific primers. Relative fold change was calculated with β-actin as reference. DMSO-treated sample value was used as one unit. Error bars represent the standard error from 3–4 independent samples. *:p<0.05; **:p<0.01; ***:p<0.001; if no * is indicated, p>0.05.