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. 2018 Oct 9;11:357. doi: 10.3389/fnmol.2018.00357

Figure 2.

Figure 2

Tmod1 and Tmod2 require their actin-binding sites to modulate dendritic morphology. Primary hippocampal neurons were co-transfected with a pCAGGs plasmid encoding ClFP-tagged Tmod, wild type (WT) or with disrupted actin-binding sites (constructs shown in (A)), and a plasmid encoding RFP-MAP2b which was used as a dendritic marker. (B–H) Representative images of neurons at 9 days in vitro (DIV), analyzed in this experiment. Shown images are RFP fluorescence signals (scale bars = 100 μm). (I–K) Imaged neurons were analyzed for number of primary dendrites (left), dendritic termini (center) and total dendritic length (right). 29–55 neurons were analyzed for each condition, data pooled from two cultures with no statistical differences between controls. Error bars indicate standard error of the mean (SEM). Asterisks and plus symbols indicate statistically significant difference from the control and overexpression of WT Tmods, respectively (Unpaired t-test for normally distributed samples, Mann Whitney test for non-normally distributed samples, **P < 0.01, ***P < 0.001, ****P < 0.0001, ++P < 0.01, +++P < 0.001, ++++P < 0.0001).