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. 2018 Oct 9;11:372. doi: 10.3389/fnmol.2018.00372

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Copyright © 2018 Zacco, Martin, Thorogate and Pastore.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Thioflavin-T emission fluorescence spectra associated with the three TDP-43 constructs, recorded at room temperature at between 460 and 600 nm, before (continuous lines) and after (dashed lines) heat treatment. The excitation wavelength was set at 440 nm. Blue: RRM1; green: RRM2; and fuchsia: RRM1-2. The reported fluorescence values are an average of three experiments, each conducted with three accumulations. The results are expressed as percentage, where 100% is the highest ThT-associated fluorescence value obtained within the assays, corresponding to RRM1-2 emission value at 485 nm after heating.