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. 2018 Sep 25;9:1336. doi: 10.3389/fphys.2018.01336

FIGURE 2.

FIGURE 2

Western blot analysis of Akt and p70S6k proteins in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes. Myotubes were infected with GFP, PGC-1α, or PGC-1β adenoviruses for 48 h, and samples were extracted after 72 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Samples were harvested after 72 h of infection. Bands were normalized to GAPDH protein. The same control images have been used for A,C, and B,D. n = 5 per group. ∗P < 0.01 vs. GFP. (E) Protein synthesis in GFP, PGC-1α, and PGC-1β infected C2C12 myotubes, treated with LY294002 (LY294) or Rapamycin and compared to basal conditions. n = 6, repeated in three experiments. ∗∗P < 0.01, ∗∗∗P < 0.001 vs. GFP within the same treatment; #P < 0.05, ###P < 0.001 vs. control within the same condition.