FIGURE 4.

Western blot analysis of Akt and p70S6k proteins in C2C12 myotubes infected with AdshERRα and PGC-1 adenoviruses. Myotubes were infected with either AdSUPER or AdshERRα for 24 h, followed by infection with GFP, PGC-1α, or PGC-1β for a further 48 h. Samples were harvested after 96 h. (A) Phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A–C. n = 4 per group. ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. GFP-AdShERRα; ^#P < 0.05, ^##P < 0.01 vs. to AdSUPER for each condition.