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Western blot analysis of Akt and p70S6k proteins in VP16-ERRα infected C2C12 myotubes. Myotubes were infected with VP16-control or VP16-ERRα adenoviruses for 48 h, and samples were extracted after 72 h. (A) phospho-Akt (ser473), (B) total Akt protein, (C) phospho-p70S6k (thr389), and (D) total p70S6k protein expression. Bands were normalized to GAPDH protein. The same control images have been used for A,B. n = 5 per group. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 vs. VP16-control.
