Figure 6.

Involvement of TLR2 in the activation of bone marrow-derived macrophages (BMDMs) by T. interdigitale EVs. BMDMs from WT, TLR2^−/−, and TLR4^−/− mice were stimulated for 6 h with EVs (10⁷ particles/mL). As positive control for the M1 activation, the mixture of INF-γ (2 ng/mL) plus IL-12p40 (50 ng/mL) was used. For M2 activation, a mixture of IL-10 plus IL-4 (50 ng/mL both) was used. The medium alone was used as negative control. RNA was extracted and converted into cDNA and real-time PCR was used to evaluate the relative expression of iNOS (A), Ym-1 (B), and arginase-1 (C). Data are representative of 3 experiments. The results are expressed as means ± SEM and are compared to the results obtained for the negative control. ***P < 0.001, and non-significant differences (n.s.).