FIGURE 3.

Western blot showing the expression of insulin signaling proteins in oxidative (SOL) and glycolytic (EDL) skeletal muscles of young rats submitted to 14 days of HFD or RCD (A). Blots were cropped for display. Quantification of IRS-1, Total Akt, phospho-Akt, Total S6, and phospho-S6 protein contents (normalized for their corresponding ponceau stain intensities) are shown in SOL (B) and EDL (C) muscles. Mann-Whitney U tests were used to compare values between both groups. Results are presented as means ± SEM for n = 4.