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. 2018 Oct 4;9:449. doi: 10.3389/fgene.2018.00449

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Copyright © 2018 Li, Zhou, Li, Li, Ding, Li, Liu, Qu, Sonstegard, Huang, Jiang, Chen, Petersen and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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p53 dependent expression in the muscles of edited animals and controls. (A) Heatmap of p53 and cell death related genes in F1 progenies and WT animals in muscle and skin tissues. (B) Immunofluorescence staining of muscles from a mutant (#040) and a control animal (#11) using anti-p53 antibodies (green) and counterstained with Hoechst 33342 (blue). Scale bar, 200 μm. (C) Western blot analysis using anti-p53 and anti-β-actin (control) antibodies in mutant (#009, #040, and #082) and three controls (#11, #13, and #15).