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. 2018 Sep 27;9:2129. doi: 10.3389/fimmu.2018.02129

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Copyright © 2018 Bazhin, von Ahn, Fritz, Werner and Karakhanova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFNα up-regulates PD-L1 expression on different myeloid immune cell populations ex vivo. FACS analysis of PD-L1 expression on the surface of different myeloid immune cells from splenocytes of healthy mice. Splenocytes were isolated, treated for 24 h with 1,000 U/ml IFNα and investigated by flow cytometry. The results are presented as interleaved bars (A,B), as box and whiskers plots (C,E) or column bar graphs (D,F) and statistically analyzed using two-way ANOVA (A,B) or unpaired T-tests (C–F), n = 4, ^**p < 0.01, and ^***p < 0.001.