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. 2018 Oct 9;9:1413. doi: 10.3389/fphys.2018.01413

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Copyright © 2018 de Michele, Poels, Vermeulen, Ambroise, Gruson, Guiot and Wyns.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Angiotensin converting enzyme (ACE), Synaptonemal complex protein 3 (SYCP3) and Protein boule-like (BOLL) expression. (a1,b1,c1) Show IHC for SYCP3 in patient 5 (aged 12 years old), at days 0, 16, and 32, respectively, with the presence of staining at day 16 (b1) and day 32 (c1). (a2,b2,c2) Show IHC for BOLL in patient 1 aged 2 years old, at days 0, 16, and 64, respectively, with the presence of staining at days 16 and 64. (a3,b3) Shows IHC for ACE in patient 3 aged 8 years old at day 0 (absence of staining) and 64 (presence of staining). (d1–d3) Shows the SYCP3, BOLL, and ACE negative controls performed on mature testicular tissue. We analyzed one slide for each patient and for each time point, for both culture media.