Table 3.
Antibodies and target cells.
| Antibody | Target cell | Dilution | Manufacturer | Reference |
|---|---|---|---|---|
| ACE | Elongated spermatids | 1/160 | BIO-RAD | MCA2056 |
| AMH | Mature Sertoli cells | 1/200 | Serotec | MCA2246 |
| AR | Mature Sertoli cells | 1/100 | DAKO | AR441 |
| BOLL | Spermatocytes and round | 1/4000 | Sigma-Aldrich | HPA 0488-13 |
| CASPASE 3 | Apoptotic cells | 1/200 | Promega | G7481 |
| GDNF | Sertoli cells | 1/150 | Sigma-Aldrich | SAB1405863 |
| Ki 67 | Proliferating cells | 1/150 | DAKO | M7240 |
| MAGEA4 | Spermatogonia | 1/500 | Given by Giulio Spagnoli | / |
| PROTAMINE 2 | Elongated spermatids | 1/2000 | Sigma-Aldrich | HPA056386 |
| SOX9 | Sertoli cells | 1/2000 | Abcam | 185966 |
| STAR | Leydig cells | 1/400 | Sigma-Aldrich | HPA027318 |
| SYCP3 | Spermatocytes | 1/4000 | Sigma-Aldrich | HPA039635 |
| TNP1 | Elongated spermatids | 1/500 | Sigma-Aldrich | HPA044387 |