Figure 3.

Myeloid Bmal1 deficiency leads to proinflammatory macrophage phenotype. A–D) Summarized data of Ly6c^hi monocyte-derived macrophages (A), Ly6c^mid macrophages (B), Ly6c^lo macrophages (C), and F4/80^hi macrophages (D) in atherosclerotic aorta. Data are means ± sem (n = 8). *P < 0.05, **P < 0.01 vs. Apoe^−/−; Bmal^f/f. E) Representative flow plots of myeloid cells (CD45⁺CD11^b) gated on F4/80 and Ly6c for monocytes and macrophages from the aorta. F) Representative immunofluorescence staining of aortic root sections (from 4 different mice per group) showing the expression of Ly6c (green), CD68 (red), and counterstain with DAPI. Scale bars, 100 μm.