Figure 6.

Glucose and palmitate induce differential mitochondrial respiratory phenotypes in DRG neurons. After stable baseline OCR measurements, mitochondrial bioenergetic profiles were determined by sequential addition of oligomycin (ATP-synthase inhibitor), FCCP [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone, uncoupler], and antimycin A (complex III inhibitor) for final concentrations of 1.25 µM, 300–1000 nM, and 1 µM, respectively. A–D) Resting mitochondrial bioenergetic phenotype. E–H) Absolute SRC (E, G), and SRC expressed as a percentage of resting basal respiration determined in A, F, and H. I) Mitochondrial copy number was assessed by normalizing mitochondrial gene expression (cyto b) to nuclear gene expression [tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein (Ywhaz)]. Data are expressed as fold-change (FC) in copy number relative to respective control. *P < 0.05, **P < 0.01, ***P < 0.001 vs. respective control, 1-way ANOVA with Tukey’s multiple-comparisons test (A–D, I) or Bonferroni multiple-comparisons post hoc text (E–H); ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, 2-way ANOVA whole-curve effect of treatment vs. control. Values are expressed as means ± sem.