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. 2018 Oct 16;16(10):e2006497. doi: 10.1371/journal.pbio.2006497

Fig 1. Paternal nicotine exposure paradigm and behavioral phenotypes in F1 male and female mice.

Fig 1

(A) Paternal exposure to nicotine in drinking water and production of F1 mice. (B) Spontaneous locomotor activity was measured in F1 mice over a 12-h period (19:00 to 07:00 h; lights-off period; F1 water male n = 18; F1 nicotine male n = 12; F1 water female n = 13; F1 female nicotine n = 11). (C) Attention was assayed using recognition index in an object-based attention test (F1 water male n = 8; F1 nicotine male n = 7; F1 water female n = 9; F1 female nicotine n = 7), and (D) spatial working memory was assayed using spontaneous alternation index in a Y-maze (F1 water male n = 12; F1 nicotine male n = 13; F1 water female n = 10; F1 female nicotine n = 10). A Barnes Maze was used to measure acquisition learning and reversal learning based on latency to escape (panel E and G) and nose poke errors (panel F and H) (F1 water male n = 8; F1 nicotine male n = 11; F1 water female n = 8; F1 female nicotine n = 11). Data were analyzed by two-way ANOVA. When main effect or interaction was significant, the ANOVA was followed by Bonferroni post hoc test. Asterisks for post hoc comparisons **p < 0.01 and *p < 0.05 (S1 Data).