TABLE 3.

Descriptive statistics of whole lung and mesenchymal tissue qPCR analysis

Gene	Whole lung			Mesenchyme
	Room air	Smoke	P	Room air	Smoke	P
Axin2	1.00 ± 0.11	0.89 ± 0.09	0.43	1.00 ± 0.18	0.73 ± 0.02	0.28
CyclinD1	1.00 ± 0.08	0.74 ± 0.08	0.05*	1.00 ± 0.07	0.61 ± 0.09	0.02*
sFRP-1	1.00 ± 0.38	2.33 ± 0.34	0.03*	1.00 ± 0.49	0.46 ± 0.13	0.27
Lef1	1.00 ± 0.29	0.70 ± 0.16	0.41	1.00 ± 0.15	1.23 ± 0.52	0.69
Bmp4	1.00 ± 0.11	0.76 ± 0.08	0.10
Wnt2				1.00 ± 0.35	1.21 ± 0.90	0.86

Tissue-specific digests were obtained by laser capture microdissection of 16.5 dpc lungs. All qPCR analyses were performed in triplicate each for 3 to 6 lungs per group. Data are presented as means ± sem. P values were determined by Student’s t test. *P < 0.05.