Table 2. Correlation of clinico-pathological chracterestics and CDO1 methylation in primary EHCC.

Factor category	Clinico-pathological parameter	CDO1 TaqMeth value
		Low (<28.9)			High (≥28.9)		p value
		No.	%		No.	%
Preoperative factor	Age	≥65	30	65	16	35	0.08
		<65	29	83	6	17
	Gender	Male	41	69	18	31	0.27
		Female	18	82	4	18
	Preoperative jaundice	absence	27	68	13	33	0.29
		presence	32	78	9	22
	Biliary drainage	absence	9	69	4	31	0.75
		presence	50	74	18	26
	Preoperative serum CA19-9	≥37	39	75	13	25	0.56
		<37	20	69	9	31
	Preoperative serum CEA	≥5	1	25	3	75	0.027
		<5	58	75	19	25
Pathological factor	Tumor location	Bp	11	61	7	39	0.20
		Bd	48	76	15	24
	Lymphatic permeation	absence	16	89	2	11	0.083
		presence	43	68	20	32
	Vascular permeation	absence	20	80	5	20	0.33
		presence	39	70	17	30
	Portal venous invasion	absence	57	74	20	26	0.29
		presence	2	50	2	50
	Arterial system invasion	absence	56	72	22	28	0.28
		presence	3	100	0	0
	Perineural invasion	absence	17	77	5	23	0.58
		presence	42	71	17	29
	Macroscopic growth pattern	invasive	52	73	19	27	0.83
		others	7	70	3	30
	Histology	tub1,pap	38	78	11	22	0.24
		others	21	66	11	34
	Resection status	R0	31	72	12	28	0.87
		R1,2	28	74	10	26
	pT	Tis,T1	9	90	1	10	0.40
		T2	10	83	2	17
		T3	21	68	10	32
		T4	19	68	9	32
	pN	absence	35	81	8	19	0.07
		presence	24	63	14	37
	pStage	0,IA,IB	14	93	1	7	0.26
		IIA	12	71	5	29
		IIB	14	67	7	33
		III	19	68	9	32
Treatment factor	Operative procedure	PD	49	77	15	23	0.19
(postoperative factor)		Liver resection	9	64	5	36
		others	1	33	2	67
	Postoperative jaundice	absence	50	71	20	29	0.47
		presence	9	82	2	18
	Postoperative serum CA19-9	≥37	11	58	8	42	0.09
		<37	48	77	14	23
	Postoperative serum CEA	≥5	2	100	0	0	0.38
		<5	57	72	22	28
	Postoperative chemotherapy	absence	15	75	5	25	0.80
		presence	44	72	17	28