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| Reagent | Amount to add |
|---|---|
| 1 m HEPES (pH 7.7, adjusted with KOH) | 5 mL |
| XB salts (20×) <R> | 25 mL |
| 0.5 m EGTA (pH 8.0, adjusted with KOH) | 5 mL |
| Sucrose | 50 g |
Combine the listed reagents in abeaker. Add β-mercaptoethanol to a final concentation of 6 mM and bring the volume to 500 mL with deionized H2O (18.2 MΩ-cm). Stir with a magnetic bar to dissolve sucrose and mix reagents. Adjust to pH 7.8 if necessary. Store at 4° C.
