KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PRMT4 (IHC, western blot, chIP) Rabbit | Millipore | 09–818, RRI D AB_1587416 |
| Asymmetric-dimethyl H3R17 (IHC, western blot, chIP) Rabbit |
Abcam | ab8284, RRID:AB_306434 |
| Asymmetric-dimethyl BAF155 Rabbit | Millipore | ABE1399 |
| BAF155 (D7F86) Rabbit | Cell Signaling | 49926 |
| Total Histone H3 (C-16) | Santa Cruz | Sc-8654, RRID:AB_2118303 |
| Mono-methyl SmB | Sigma Aldrich | S0698, RRID:AB_2193867 |
| Asymmetric-Methyl-PABP1 (Arg455/Arg460) (C60A10) | Cell Signaling | #3505, RRID:AB_2298971 |
| Anti-PABP antibody [10E10] | Abcam | Ab6125, RRID:AB_2156878 |
| GAPDH FL-335 Rabbit | Santa Cruz | Sc-25778, RRID:AB_10167668 |
| Donkey Anti-Mouse IgG 680RD | LI-COR- Biosciences | 926–68072 RRID:AB_10953628 |
| Donkey Anti-Rabbit IgG 680RD | LI-COR- Biosciences | 926–68073, RRID:AB_10954442 |
| Donkey Anti-Mouse IgG 800CW | LI-COR- Biosciences | 926–32212, RRID:AB_621847 |
| Donkey Anti-Rabbit IgG 800CW | LI-COR- Biosciences | 926–32213, RRID:AB_621848 |
| Biotin-CD3e | BD Biosciences | 553059, RRID:AB_394592 |
| Biotin-CD4 | BD Biosciences | 553728, RRID:AB_395012 |
| Biotin-CD5 | BD Biosciences | 553019, RRID:AB_394557 |
| Biotin-CD8 | BD Biosciences | 553029, RRID:AB_394567 |
| Biotin-CD127 | BD Biosciences | 555288, RRID:AB_395706 |
| Biotin-Gr-1 | BD Biosciences | 553125, RRID:AB_394641 |
| Biotin-B220 | BD Biosciences | 553086, RRID:AB_394616 |
| Biotin-Ter119 | BD Biosciences | 553672, RRID:AB_394985 |
| APC-c-Kit | BD Biosciences | 553356, RRID:AB_398536 |
| Strep-PerCP-Cy5.5 | BioLegend | 405214, RRID:AB_2716577 |
| PE-CD71 | Biolegend | 113808, RRID:AB_313569 |
| FITC-Ter119 | Biolegend | 116206, RRID:AB_313707 |
| FITC-Annexin-V | Biolegend | 640905, RRID:AB_2561291 |
| PE-cy7-Sca-1 | Thermo Fisher Scientific |
255981, RRID:AB_469668 |
| APC-Mac-1 | Thermo Fisher Scientific |
17–0112, RRID:AB_469343 |
| Biological Samples | ||
| Human: Umbilical cord blood for CD34 purification | NYBB nybloodcenter.org |
N/A |
| Human: AML064 | TCBF-UM | TCBF-AML064 |
| Human: AML065 | TCBF-UM | TCBF-AML065 |
| Human: AML066 | TCBF-UM | TCBF-AML065 |
| Human: AML067 | TCBF-UM | TCBF-AML065 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Poly (I:C) HMW | Invivogen | Tlrl-pic-5 |
| EPZ025654/GSK3536023 CARM1 tool compound | Epizyme | N/A |
| EZM2302/GSK3359088 CARM1 in vivo compound | Epizyme | N/A |
| EPZ029751 control compound | Epizyme | N/A |
| M3434 Methylcellulose, Mouse | STEMCELL technologies |
03434 |
| H4435 Methylcellulose, Human | STEMCELL technologies |
04435 |
| Methyl cellulose viscosity 400 cP (In vivo vehicle) | Sigma-Aldrich | M0262 |
| Critical Commercial Assays | ||
| CellTiter-Glo | Promega | G7572 |
| CD34 Microbead Kit, Human | Miltenyi | 130–046-702 |
| Senescence β-Galactosidase Staining Kit | Cell Signaling | #9860 |
| RNeasy Mini Kit | Qiagen | #74106 |
| Deposited Data | ||
| RNA-sequencing: CARM1 knockdown in AML cell lines | This paper | GEO: GSE103528 |
| Experimental Models: Cell Lines | ||
| Human: AML-14 | OSU | RRID:CVCL_8286 |
| Human: HEL | ATCC | TIB-180, RRID:CVCL_2481 |
| Human: HL-60 | ATCC | CCL-240, RRID:CVCL_A794 |
| Human: KASUMI-1 | ATCC | CRL-2724, RRID:CVCL_0589 |
| Human: MOLM-13 | DSMZ | ACC-554, RRID:CVCL_RS50 |
| Human: MV4–11 | ATCC | CRL-9591, RRID:CVCL_0064 |
| Human: NB-4 | DSMZ | ACC-207, RRID:CVCL_0005 |
| Human: NOMO-1 | DSMZ | ACC-542. RRID:CVCL_1609 |
| Human: OCI-AML3 | DSMZ | ACC-582, RRID:CVCL_1844 |
| Human: SET-2 | DSMZ | ACC-608, RRID:CVCL_2187 |
| Human: SKNO-1 | DSMZ | ACC-690, RRID:CVCL_2196 |
| Human: TF-1 | ATCC | CRL-2003, RRID:CVCL_0559 |
| Human: THP-1 | ATCC | TIB-202, RRID:CVCL_0006 |
| Human: U937 | ATCC | CRL-1593.2, RRID:CVCL_0007 |
| Human: UKE-1 | Coriell | RRID:CVCL_4Z62 |
| Experimental Models: Organisms/Strains | ||
| Mouse: CARM1 conditional knockout | Laboratory of Dan Tenen |
N/A |
| Mouse: B6.SJL-Prprca PEPCb/BoyJ Age: 6–8 weeks |
The Jackson Laboratory |
Stock: 002014 |
| Mouse: Vav1-cre B6.Cg-Tg(Vav1-cre) A2Kio/J | The Jackson Laboratory |
Stock: 008610 |
| Mouse: Mx1-cre B6.Cg-Tg(Mx1-cre) 1Cgn/J | The Jackson Laboratory |
Stock: 003556 |
| Mouse: NOD.Cg-Prkdcscid
Il2rgtm1Wjl Tg(CMV- IL3,CSF2,KITLG)1Eav/MloySzJ |
The Jackson Laboratory |
Stock: 013062 |
| Oligonucleotides | ||
| Human CARM1-KD1- CTATGGGAACTGGGACACTTT | RNAi Core MSKCC Moffat 2006 |
N/A |
| Human CARM1KD2- CGATTTCTGTTCCTTCTACAA | RNAi Core MSKCC Moffat 2006 |
N/A |
| Human AML1-ETO-KD1- GCAAGTTGAAACGCTTTCTTA |
Sigma-Aldrich | TRCN0000013366 |
| Human AML1-ETO-KD2- GCGCTGAATTGCATTATGGAA |
Sigma-Aldrich | TRCN0000013367 |
| See Table S6 for Taqman primer sequences | ||
| Recombinant DNA | ||
| MIGR1 plasmid-GFP | Addgene Pear 1998 | #27490 |
| MIGR1-AE9a-GFP | Addgene Yan 2006 | #12433 |
| MIGR1-MLL-AF9-GFP | Addgene Saito 2015 | #71443 |
| pCDH-PRMT4-WT-RFP | This paper | N/A |
| pCDH-PRMT4-EQ-RFP | This paper | N/A |
| pLKO1.1 TRC Cloning vector | Addgene Moffatt 2006 | #10878 |
| Software and Algorithms | ||
| Dep map |
depmap.org Tsherniak 2017 |
N/A |
| FlowJo Version 9.3.3 | TreeStar | N/A |
| Other | ||