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. 2018 Oct 10;4(10):eaat2681. doi: 10.1126/sciadv.aat2681

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 3 — (A to D) Cerebellar sections prepared from PTPMT1^+/+/Nestin-Cre⁺ and PTPMT1^fl/fl/Nestin-Cre⁺ mice at the indicated ages were processed for immunofluorescence staining with the indicated antibodies, followed by DAPI counterstaining. Representative images from three mice per group are shown. (E) Cerebellar cortical tissues dissected from PTPMT1^+/+/Nestin-Cre⁺ and PTPMT1^fl/fl/Nestin-Cre⁺ newborn pups (P0) and embryos at E17.5 were processed for microexplant culture. Seventy-two hours later, tissues and cells on the coverslips were processed for immunofluorescence staining for Nestin and GFAP, followed by DAPI counterstaining.