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. 2018 Jun 4;25(5):465–476. doi: 10.1093/dnares/dsy017

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© The Author(s) 2018. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Silencing of LNC1/2 in sea buckthorn fruit. (A) Sea buckthorn fruits (3 weeks after infiltration). CK: sea buckthorn fruits infiltrated with pTRV1 and pTRV2; TRV-LNC1: sea buckthorn infiltrated with pTRV1 and pTRV2-LNC1; TRV-LNC2: sea buckthorn infiltrated with pTRV1 and pTRV2-LNC2. (B) High-performance liquid chromatography analysis of total anthocyanin content in sea buckthorn fruits. (C) qRT -PCR analysis of the expression of related genes in sea buckthorn fruits. The expression of 18S rRNA was used as an internal reference. The relative level was normalized to that in TRV control plants. Error bars indicate SD of three biological replicates, each sample measured in three replicates. Asterisks indicate a significant difference as determined by Student's t-test (*P < 0.01).