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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Cancer Res. 2018 Sep 5;78(20):5877–5890. doi: 10.1158/0008-5472.CAN-18-1011

Figure 4. EGR1 enhances GC cell proliferation.

Figure 4.

(A) EdU assay was performed to observe the effect of EGR1 overexpression on cell proliferation.

(B). Cells were seeded in 96-well plates after transfection with EGR1 overexpression or control vector, and cell number was determined every 24 h using MTS assays.

(C). Effect of EGR1 overexpression on the colony formation of MKN-45 and BGC-823 cells.

(D). Effect of EGR1 overexpression on the cell cycle progression of both cells.

(E). The expression levels of CDK2, CDK4, cylcinE1 and P21 in both MKN-45 cells and BGC-823 cells transfected with EGR1 overexpression vector were detected by western blotting assay.

(F). Apoptosis was determined by flow cytometry, which showed that EGR1 did not induce apoptosis in comparison with the control cells. Three independent experiments were performed, and data are shown as mean ± SD.

*P < 0.05, ** P < 0.01, *** P < 0.001.