B6 mice were infected with MCMVΔm157 at either low dose (102 pfu) or high dose (104 pfu) to induce NK memory, and D+/R+ transplants were performed as in Figure 3. Memory NK responses from low dose and high dose infected recipients were compared to primary NK responses in D+/R− transplants.
(A, B) Allograft IFN-γ+ NK cells (A) or GzB+ NK cells (B) were compared between D+/R−, D+/R+ low dose (102) and D+/R+ high dose (104) infected recipients.
(C) Spleens from pre-transplant low dose (102) or high dose (104) MCMV+ B6 mice were analyzed for pre-transplant baseline IFN-γ+ and GzB+ NK cells.
(D, E) Splenic IFN-γ+ NK cells (D) or GzB+ NK cells (E) were compared between D+/R−, D+/R+ low dose (102) and D+/R+ high dose (104) infected recipients.