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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Am J Transplant. 2018 May 7;18(11):2647–2662. doi: 10.1111/ajt.14868

FIGURE 7. MCMV D+/R+ different-strain infection influences NK and T cell responses to MCMV infected allografts.

FIGURE 7

B6 recipients were infected with 104 pfu of MCMV Smith wild-type (MCMV WT) strain > 12 weeks prior to transplant, and received BALB kidneys infected with MCMVΔ157 strain (104 pfu). At day 14 post-transplant, allograft viral loads, NK and T cell infiltrates were analyzed in comparison to same-strain D+/R+ transplants (104 pfu), as well as D+/R+ same-strain transplants with low dose (102 pfu) recipient infection.

(A) Allograft viral loads were compared between different-strain and same-strain transplants for recipients with high dose (104 pfu) primary infection.

(B) DNA was extracted from allografts of D+/R+ different-strain transplants and analyzed by quantitative PCR for MCMV using either a primer/probe set amplifying both MCMVΔ157 and MCMV WT strains (“Pan-MCMV PCR,” grey bar) or a primer/probe set amplifying only MCMV WT virus (“WT-Only PCR,” white bar).

(C–E) D+/R+ transplants with recipient same-strain virus at low dose (102) or high dose (104), or different-strain virus at high dose (104), were compared for allograft-infiltrating (C) IFN-γ+ NK cells, (D) GzB+ NK cells, or (E) TNF-α+ NK cells.

(F–H) Allograft-infiltrating (F) IFN-γ+ CD4+ T cells (Th1); (G) IL-17A+ CD4+ T cells (Th17), or (H) IFN-γ+ CD8+ T cells were compared between the three D+/R+ groups.

(I) Allograft-infiltrating MCMV M45/M38 peptide-specific IFN-γ+ CD8+ T cells were compared between D+/R+ (104 pfu) same-strain and different-strain virus recipients.