Table 1.
List of strains used in this study.
| USA300 LAC | S. aureus USA300 Strain LAC (AH-1264). Plasmid cured. | AH-LAC (WT) | (Boles et al., 2010) |
| DC10B | E. coli strain for recombinant pJC plasmids (dcm-) | DC10B | (Monk et al., 2012) |
| lysY/Iq | E. coli lysY/Iq for expression of LplA1 and LplA2 | lysY/Iq | NEB |
| FA-E1344 | E. coli lysY/Iq ΔlipA::kan for expression of H and E2 subunits | lysY/Iq ΔlipA::kan | (Grayczyk et al., 2017) |
| RN4220 | Restriction deficient S. aureus for plasmid passage | RN4220 | (Fairweather et al., 1983) |
| RN9011 | RN4220 + pRN7203 expressing SaPI integrase | RN9011 | (Chen et al., 2014) |
| FA-S1249 | AH-LAC with in-frame deletions of lipA and lplA1 | ΔlipAΔlplA1 | (Zorzoli et al., 2016) |
| FA-S1180 | AH-LAC with in-frame deletions of lipA and lplA2 | ΔlipAΔlplA2 | (Zorzoli et al., 2016) |
| FA-S1178 | AH-LAC with in-frame deletions of lipA, lplA1, and lplA2 | ΔlipAΔlplA1ΔlplA2 | (Zorzoli et al., 2016) |
| FA-S1200 | FA-S1178 complemented with pJC1111-lplA1 | ΔlipAΔlplA1ΔlplA2+lplA1 | (Zorzoli et al., 2016) |
| FA-S1212 | FA-S1178 complemented with pJC1111-lplA2 | ΔlipAΔlplA1ΔlplA2+lplA2 | (Zorzoli et al., 2016) |
| FA-S953 | AH-LAC ΔlipL::kan | ΔlipL::kan | (Zorzoli et al., 2016) |
| FA-S698 | JE2 lipM::erm transposon insertion mutant (NE1334) | lipM::erm | (Zorzoli et al., 2016) |
| FA-S1476 | AH-LAC ΔlipA ΔlplA1 ΔlplA2 ΔlipL::kan lipM::erm | ΔlipAΔlipMΔlipLΔlplA1ΔlplA2 | This work |
| FA-S1038 | AH-LAC with gene replacement of gcvH | ΔgcvH::kan | (Zorzoli et al., 2016) |
| FA-S1637 | AH-LAC ΔgcvH::kan containing pJC1111-gcvH | ΔgcvH::kan+gcvH | This work |
| FA-S1582 | AH-LAC ΔgcvH::kan containing pJC1111-gcvH-L | ΔgcvH::kan+gcvH-L | This work |
| FA-S1636 | AH-LAC ΔgcvH::kan containing pJC1111-gcvH-L (K56A) | ΔgcvH::kan+gcvH-L* | This work |
| FA-S1435 | AH-LAC with in-frame deletion of gcvH-L | ΔgcvH-L | This work |
| FA-S1498 | AH-LAC ΔgcvH-L containing pJC1111-gcvH-L | ΔgcvH-L + gcvH-L | This work |
| FA-S1645 | AH-LAC with gene replacement of gcvH and in-frame deletion of gcvH-L | ΔgcvH::kan ΔgcvH-L | This work |
| FA-S1684 | AH-LAC ΔgcvH::kan ΔgcvH-L containing pJC1111-gcvH | ΔgcvH::kan ΔgcvH-L + gcvH | This work |
| FA-S1686 | AH-LAC ΔgcvH::kan ΔgcvH-L containing pJC1111-gcvH-L | ΔgcvH::kan ΔgcvH-L + gcvH-L | This work |
| FA-E1357 | E. coli lysY/Iq ΔlipA::kan + pET15b-6x-His-gcvH | 6x-His-GcvH | This work |
| FA-E1383 | E. coli lysY/Iq ΔlipA::kan + pET15b-6x-His-gcvH-L | 6x-His-GcvH-L | This work |
| FA-E1359 | E. coli lysY/Iq ΔlipA::kan + pET15b-6x-His-e2-pdh | 6x-His-E2-PDH | This work |
| FA-E1363 | E. coli lysY/Iq ΔlipA::kan + pET15b-6x-His-e2-ogdh | 6x-His-E2-OGDH | This work |
| FA-E1367 | E. coli lysY/Iq ΔlipA::kan + pET15b-6x-His-e2-bcodh | 6x-His-E2-BCODH | This work |
| FA-E1284 | E. coli lysY/Iq + pET15b-6x-His-lplA1 | 6x-His-LplA1 | This work |
| FA-E1278 | E. coli lysY/Iq + pET15b-6x-His-lplA2 | 6x-His-LplA2 | This work |