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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Shock. 2019 Apr;51(4):453–463. doi: 10.1097/SHK.0000000000001163

FIG. 6. Effect of alcohol and sepsis on CD8+ T cells.

FIG. 6

Both alcohol in isolation (p<0.0001) and sepsis in isolation decreased frequency of CD8+ T cells (p=0.048). Further, CD8+ T cell frequency was lower in alcohol-fed septic mice than water-fed septic mice (A, p=0.012). Representative flow cytometry plot for CD8+ T cells is shown in figure 5D. Sepsis in isolation also decreased frequency of central memory CD8+ T cells in both water-fed (p=0.002) and alcohol-fed mice (p=0.004), but there was no difference in central memory CD8+ T cell frequency between alcohol-fed septic mice and water-fed septic mice (B, p=0.41). Effector memory CD8+ T cell frequency was similar in all groups (C, p=0.99 comparing alcohol-septic to water-septic). Representative flow cytometry plot for both central memory and effector memory CD8+ T cells is shown (D). Stimulated cytokine production from CD8+ T cells showed increased TNF (E, p<0.0001) and IFNγ (F, p=0.02) in alcohol-septic mice compared to water-septic mice without a change in IL-2 (G, p=0.98), n=8-11/group for all panels. Neither anti-TNF nor anti-IFNγ altered mortality following alcohol ingestion and sepsis compared to isotype control (H, p=0.49 and p=0.66 respectively). n=8-11/group for all panels except survival where n=14-18/group.