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. 2018 Oct 16;8:15286. doi: 10.1038/s41598-018-33306-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gas1 is required for microglia-induced BTIC growth reduction. (A) The Gas1 protein level in BT048 was decreased by 2 of 3 siRNAs, as evident through western blot analysis and corroborated by FACS. The gels were cut at the relevant molecular weight to highlight the bands of interest. (B) Cell counts showed that MCM exposure reduced the number of BT048 cells and this was abrogated in siRNA-treated, Gas1-reduced cultures. In the absence of MCM, the Gas1 targeted siRNAs and control siRNA did not affect BTIC growth (n of 4 where error bar represents sem, ***p < 0.001, with ANOVA comparison to MCM or control siRNA; note that statistical comparisons are only displayed for the MCM conditions). (C) Unlike MCM collected from control individuals, GBM-MCM had no effect on Gas1 siRNA-transfected cells. N of 4 where error bars represent sem; ***p < 0.001 compared to all groups.