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. 2018 Oct 16;9:4292. doi: 10.1038/s41467-018-06230-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The SGET reporter for tracking cell fate transitions toward PGCs. a Single-cell RNA-seq analysis showing reciprocal gene expression patterns of endogenous Stella and Esg1 during early development (ICM; E3.5 or Epiblast; E6.5) and in primordial germ cells (PGC). b Chimera and tetraploid complementation assays confirm faithful expression of the Stella-GFP (green) and Esg1-tdTomato (red) SGET reporter during early development and in PGCs. c Representative FACS analysis of SGET activation during in vitro cell fate transitions of ESC into EpiLC and subsequently PGCLC. d Representative images of impaired PGCLC induction from Blimp1^−/− SGET ESC. Numbers indicate percentage of SG⁺ET^low PGCLC at day 5 as determined by FACS. Scale bar(s): 200 µm