Fig. 3.

Candidate genes for primordial germ cell fate. a Heatmap showing mean fold-change (FC) in normalised frequency of gRNAs that target candidate PGC regulators, relative to their frequency in the preceding cell state. b Log₂ RPKM gene expression dynamics of candidate PGC regulators during induction of SGET ESC into d6 PGCLC in the screen. c Normalised frequency of individual gRNAs targeting Nr5a2 and Zfp296 during induction of PGCLC. d Expression dynamics of Nr5a2 and Zfp296 during in vivo formation of PGCs by single-cell RNA-seq. Blimp1^−/− PGC (BKO) and soma are shown for reference