Fig. 1.

CD8⁺ TILs in breast tumors are predominantly effector memory cells. a Memory phenotypes of CD8⁺ T cells in patient peripheral blood mononuclear cells (bcPBMCs), breast cancer patient primary tumors (bcTumor), and melanoma patient tumors (melTumor) were phenotypically characterized by flow cytometry. Representative contour plots show cell population gates for naive (CCR7⁺ CD45RA⁺), central memory (CM, CCR7⁺ CD45RA⁻), effector memory (EM, CCR7⁻ CD45RA⁻), or effector memory RA⁺ (EMRA, CCR7⁻ CD45RA⁺). b Graph depicts percentage of naive, CM, EM, and EMRA among CD8⁺ T cells in bcPBMCs, bcTumor and melTumor. Each symbol represents data from a unique patient sample. Significance was calculated using one-way ANOVA and Holm–Sidak multiple comparison tests; **p < 0.01; ****p < 0.0001