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. 2018 Oct 10;8:376. doi: 10.3389/fonc.2018.00376

Figure 2.

Figure 2

Abundant production of ABCG1 and EV, but low level of Ki67 in tumoroids. (A) Representative morphologies (top) and protein concentration (bottom) in tumoroids of LuM1 cultured in 2D/serum, 3D/serum, and 3D/stem conditions. n = 3, *P < 0.05, **P < 0.01, ***P < 0.005. Similar data were obtained from at least two independent experiments. (B) Western blot showing ABCG1 in tumoroids developed in 3D/stem, 3D/serum, and 2D/serum conditions. β-actin, loading control. Proteins per 10∧5 cells were loaded. (C) Immunohistochemistry showing ABCG1 and Ki67 in tumoroids. Cell nuclei were counterstained with hematoxylin. Scale bars, 50 μm. (D,E) Concentration of EVs in conditioned medium of aggregated LuM1 and Colon26 cells. EVs were prepared from serum-starved conditioned medium for 1 or 2 days. EV protein concentrations per 106 cells (D) and per cellular protein concentration (E) were measured. n = 4, *P < 0.05, **P < 0.01.