FIGURE 2.

BX795 is a PDPK-1 inhibitor that enhances rod photoreceptor precursor development. In P1 retinal explant cultures, PDPK-1 was inhibited with 0, 0.1, 1, 5, 10, 20, and 40 μM BX795. (A) Quantification of rhodopsin labeling intensity as a function of log₁₀ (BX795 concentration), relative to untreated controls. (B) Protein levels of p-AKT1Thr308 as a function of BX795 concentration. The inset contains an example western blot with duplicate lanes for each concentration of BX795 (n = 3). (C) Representative images for the curve in A, 96 h retinal explants were sectioned and labeled for rhodopsin (green) and the DNA stain Hoechst-33258 (blue). (D) cDNA levels corresponding to the expression of photoreceptor and photoreceptor precursor-related genes. n = 3 biological replicates/measure. ^∗p < 0.05 and ^∗∗p < 0.01.