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. 2018 Oct 10;6:134. doi: 10.3389/fcell.2018.00134

FIGURE 5.

FIGURE 5

Inhibition of P70-S6K with PF4708671 enhances rod photoreceptor development. (A) Representative rhodopsin (green) and Hoechst-33258 (blue) labeling in retinal explants following a 96 h exposure to nothing (control), 100 nM BX795, 200 nM PF4708671, BX795 and PF4708671, 100 nM Go7874, or Go7874 and PF4708671. (B,C) Quantification of treatment groups represented in (A). (D) Example western blots and densitometric analysis of retinal explants left untreated or treated with 10 nM Rapamycin for 30 min. Lysate from these retinas was labeled with antibodies to detect P70-S6K Thr252, P70-S6K Thr389, and β-actin. (E) The same proteins were detected in lysate from retinal explants treated with 100 nM BX795 for 30 min. n = 3 biological replicates/measure. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.