Table 2.
Antibodies used in this study.
| Target | Host species | Application | Dilution | Catalog no. | Supplier |
|---|---|---|---|---|---|
| Akt-1 | Rabbit | WB | 1:1000 | Sc-5298 | Santa Cruz |
| p-AKT-1 (Thr308) | Rabbit | WB | 1:1000 | 2965 | Cell signaling |
| β-Actin (Clone AC-15) | Mouse | WB | 1:2500 | A1978 | Sigma-Aldrich |
| P70-S6K | Rabbit | WB | 1:750 | 9202 | Cell signaling |
| p-P70-S6K (Thr252) | Rabbit | WB | 1:1000 | ab59208 | Abcam |
| p-P70-S6K (Thr389) | Rabbit | WB | 1:1000 | 9205 | Cell signaling |
| PCNA (PC10) | Mouse | IF | 1:1000 | 2586 | Cell signaling |
| PDPK-1 | Rabbit | WB | 1:1000 | 3062 | Cell signaling |
| PDPK-1 | Mouse | IF | 1:1000 | MA5-15797 | Thermo |
| p-PDPK-1 (Ser241) | Rabbit | IF | 1:1000 | PA1-14336 | Thermo |
| p-PDPK-1 (Ser241) | Rabbit | WB | 1:1000 | 3438 | Cell signaling |
| Rhodopsin (RetP1) | Mouse | IF | 1:100 | (29) | |
| STAT3 | Rabbit | WB | 1:1000 | 12640S | Cell signaling |
| p-STAT3 (Tyr 705) | Rabbit | WB | 1:500 | 9131S | Cell signaling |
| Anti-mouse, HRP-linked | Goat | WB | 1:2000–10000 | 115-035-068 | Jackson ImmunoResearch |
| Anti-rabbit, HRP-linked | Goat | WB | 1:2000–10000 | 1110-35-144 | Jackson ImmunoResearch |
| Anti-mouse, AF488-linked | Goat | IF | 1:600 | A11001 | Life Technologies |
| Anti-rabbit, AF488-linked | Goat | IF | 1:600 | A11008 | Life Technologies |
| Anti-mouse, AF594-linked | Goat | IF | 1:600 | A11005 | Life Technologies |
| Anti-rabbit, AF594-linked | Goat | IF | 1:600 | A11012 | Life Technologies |