Table 2.

Assay reproducibility and robustness testing.

Run	Plate	Z′	% CV DMSO	% CV Niclosamide	S/B
1	1	0.82	1	6	23
	2	0.94	1	7	23
2	1	0.90	3	16	22
	2	0.90	2	7	23
3	1	0.50	13	15	18
	2	0.96	11	15	18
Mean		0.84	5	11	21
SD		0.16	5	4	2

The final assay was run in three independent runs containing two plates each of minimum signal (2% DMSO), maximum signal (25 μM niclosamide), and niclosamide CRCs with positive and negative controls. Nutrient-starved bacteria were exposed to compounds for 48 h, alamarBlue^® was added, and RFU measured after 24 h. Z′, % CV, and signal/background (S/B) ratio were calculated for each experimental run.