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. 2018 Oct 10;9:2293. doi: 10.3389/fimmu.2018.02293

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Copyright © 2018 Knuhr, Langhans, Nyenhuis, Viertmann, Kildemoes, Doenhoff, Haas and Schramm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IPSE/alpha-1-triggered basophil IL-4 inhibited the release of IL-1β (A), IL-6 (B), and TNF-α (C) from LPS-activated monocytes in the context of whole PBMC. Inhibition correlated with IL-4 (D) and IL-13 (E) release from basophils. The experiment shown in Figure 1 was repeated with PBMC instead of purified monocytes. Data for PBMC are presented as mean ± SEM, n = 4; (TNF-α: n = 3). Statistics were performed with the paired t-test; significance was shown vs. LPS without additions; ns, not significant, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, and ^****p < 0.0001.