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. 2018 Oct 10;9:2293. doi: 10.3389/fimmu.2018.02293

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Copyright © 2018 Knuhr, Langhans, Nyenhuis, Viertmann, Kildemoes, Doenhoff, Haas and Schramm.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Culture of LPS-activated monocytes in supernatant from IPSE/alpha-1-stimulated basophils resulted in inhibition of pro-inflammatory cytokine release and increased numbers of CD206- and CD209-positive monocytes. IL-1β (A) and IL-6 (B) release from monocytes, and CD206 (C) and CD209 (D) expression on monocytes cultured with supernatant from basophils (BaSN). Monocyte-derived cytokine release was normalized to the maximum release following LPS activation (100%). Data are presented as mean ± SEM, n = 9 (anti-IgE: n = 5). Statistics were performed by paired t-test; significance was shown for “LPS with BaSN” vs. “LPS without BaSN” (black bar); ^*p < 0.05, ^***p < 0.001, ^****p < 0.0001.