Figure 11.

DMRT5 and EMX2 bind a Gsx2 ventral-specific telencephalon enhancer. A, UCSC genome browser view of the Gsx2 locus with the location of the two cloned fragments tested in transgenic embryos. The identified putative DMRT3/5 and EMX2 BSs are shown. B, Left, A lateral view and a coronal section of the head of a E12.5 Gsx2 1.8 kb enhancer-LacZ reporter transgenic embryo (Construct A). Scale bars: lateral view, 500 μm; coronal section, 200 μm. Dashed line indicates the level of the section. Right, A coronal section of the brain of a Gsx2 1.3 kb enhancer-GFP reporter transgenic embryo (Construct B) processed by DAB immunostaining for GFP and a high-magnification view of the LGE region processed by IF for both GSX2 (red) and GFP (green). C, EMSA showing in vitro binding of cellular extracts containing DMRT3, DMRT5, and EMX2 or control extracts to BS3 of the Gsx2 enhancer. DMRT3/5 and EMX2 complex formation is competed by WT enhancer oligonucleotides but not by oligonucleotides containing mutations in the DMRT and EMX2 BSs. Arrowhead indicates a nonspecific band.