Figure 6.

Expression of GABAergic, glutamatergic, and OB interneuron markers in the cortex of E18.5 Dmrt3 and Dmrt5 single KO and in double KO embryos. a, o, Sagittal sections through brains of the indicated genotypes processed by ISH or IF for the indicated markers. p, The line in the schematic of the brain of WT and double KO embryos indicates the positions of the sections. For the double KO brains, two sections are shown for each marker. e′, j′, A high magnification of the Math2 and Gad67 staining in the residual cortex of the double KO. There is slight upregulation of Gad67 in both single and double mutants (*) and a dramatic loss of Math2 in the cortex of the double KO embryos. TH-positive cells that are not correctly laminated and form a large cluster in the OB-L structures of the double KO that extend more caudally in the dorsal telencephalon than in WT embryos (arrowheads). Ctx, Cortex; MFB, medial forebrain bundle; Acbn, accumbens nucleus. Scale bars: 500 μm; h, p, 50 μm. OB, olfactory bulb; OB-L, olfactory bulb like.