Table 2.
Antibodies used for double-labeling immunofluorescence histochemistry
| Combination | Primary antibody | Concentration | Vendor | Secondary antibody | Concentration | Vendor |
|---|---|---|---|---|---|---|
| CCL2 + GFAP | Goat anti-CCL2 | 1:100 | Catalog #sc-1785, Santa Cruz Biotechnology | Bio-horse anti-goat IgG + TSA fluorescein | 1:100 | Vector |
| Mouse anti-GFAP | 1:50 | Catalog #MAB3402, EMD Millipore | Cy3-donkey anti-mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCL2 + Iba-1 | Goat anti-CCL2 | 1:100 | Catalog #sc-1785, Santa Cruz Biotechnology | Bio-horse anti-goat IgG + TSA fluorescein | 1:100 | Vector |
| Rabbit anti-Iba-1 | 1:100 | Catalog #019-19741, Wako Chemicals | Cy3-donkey anti-rabbit IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCR2 + GFAP | Goat anti-CCR2 | 1:100 | Catalog #sc-6228, Santa Cruz Biotechnology | FITC-donkey anti-goat IgG | 1:100 | Jackson ImmunoResearch Laboratories |
| Mouse anti-GFAP | 1:50 | Catalog #MAB3402, EMD Millipore | Cy3-donkey anti-mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCR2 + Iba-1 | Goat anti-CCR2 | 1:100 | Catalog #sc-6228, Santa Cruz Biotechnology | FITC-donkey anti-goat IgG | 1:100 | Jackson ImmunoResearch Laboratories |
| Rabbit anti-Iba-1 | 1:100 | Catalog #019-19741, Wako Chemicals | Cy3-donkey anti-rabbit IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCR2 + MCH | Goat anti-CCR2 | 1:50 | Catalog #sc-6228, Santa Cruz Biotechnology | Cy3-donkey anti-goat IgG | 1:100 | Jackson ImmunoResearch Laboratories |
| Rabbit anti-MCH | 1:200 | Catalog #H-070-47, Phoenix Pharmaceuticals | FITC-donkey anti-rabbit IgG | 1:50 | Jackson ImmunoResearch Laboratories | |
| CCL2 + NeuN | Goat anti-CCL2 | 1:100 | Catalog #sc-1785, Santa Cruz Biotechnology | Bio-horse anti-goat IgG + TSA fluorescein | 1:100 | Vector |
| Mouse anti-NeuN | 1:50 | Catalog #MAB377, EMD Millipore | Cy3-donkey anti-mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCL2 + CCR2 | Goat anti-CCL2 | 1:100 | Catalog #sc-1785, Santa Cruz Biotechnology | Bio-horse anti-goat IgG + TSA fluorescein | 1:100 | Jackson ImmunoResearch Laboratories |
| Mouse anti-CCR2 | 1:50 | Catalog #sc-74490, Santa Cruz Biotechnology | Cy3-donkey anti-mouse IgG | 1:100 | Jackson ImmunoResearch Laboratories | |
| CCL2 + MCH | Goat anti-CCL2 | 1:100 | Catalog #sc-1785, Santa Cruz Biotechnology | Bio-horse anti-goat IgG + TSA fluorescein | 1:100 | Vector |
| Rabbit anti-MCH | 1:200 | Catalog #H-070-47, Phoenix Pharmaceuticals | Cy3-donkey anti-rabbit IgG | 1:100 | Jackson ImmunoResearch Laboratories |