FIG 3.

BadL-dependent phenotypes are restored upon deletion of badM, but not overexpression of aadR. Cells were grown in biological triplicate photoheterotrophically on benzoate (3 mM), and growth was monitored at OD₆₆₀. (A) A ΔbadM ΔbadL strain grew similar to the badL⁺ strain (red circles versus asterisks), and ectopic expression of badM⁺ in the ΔbadM ΔbadL strain restored repression, hence a lack of growth (triangles). (B) Overexpression of aadR in a ΔbadL strain did not recover growth (white triangles). Each experiment was performed in triplicate, and a representative growth curve is shown. 4-ABA was present in the growth medium at 11 µM (2 mg/liter).