TABLE 1.

Expression of neuron-specific genes in HeLa, HTB10, and SH-SY5Y cell lines

Gene	Gene product name	Expression (TPM) in linea :
		HeLa	HTB10	SH-SY5Y
STMN2	Stathmin 2	0.0	0.0	1,322.5
TCEAL7	Transcription elongation factor A-like 7	0.0	0.0	248.5
RGS4	Regulator of G-protein signaling 4	0.0	0.0	163.7
ISL1b	Insulin gene enhancer protein 1	0.0	0.0	55.7
NNAT	Neuronatin	0.0	0.0	45.8
VIP	Vasoactive intestinal peptide	0.0	0.0	39.3
TAGLN3	Transgelin 3	0.0	0.0	39.0
SNAP25	Synaptosome-associated protein 25	1.8	0.7	24.6
LMO1	LIM domain only 1	0.6	0.0	17.0
CAMK2N1	Calmodulin-dependent protein kinase II inhibitor 1	1.2	0.0	11.8
SCG2	Secretogranin II	0.0	0.0	10.7
CDO1	Cysteine dioxygenase type 1	0.0	0.0	6.9
SLC10A4	Solute carrier family 10 member 4	0.0	0.0	3.1
DLX5	Distal-less homeobox 5	0.0	0.0	2.1
DBH	Dopamine β-hydroxylase	0.0	0.0	1.7
SYT17	Synaptotagmin 17	0.1	0.0	1.1
CUX2	Cut-like homeobox 2	0.0	0.1	1.1
CNTN4	Contactin 4	0.0	0.2	1.0
DPYSL5	Dihydropyrimidinase-like 5	0.0	0.2	1.0
SV2C	Synaptic vesicle glycoprotein 2C	0.0	0.0	0.7
ETV1	ETS variant 1	0.0	0.1	0.7
DCN	Decorin	0.0	7.9	0.6
ISL2b	Insulin gene enhancer protein 2	0.0	0.0	0.3
NXPH1	Neurexophilin 1	0.0	0.0	0.1
FOXP2	Forkhead box P2	0.2	2.2	0.0
GAD1	Glutamate decarboxylase 1	0.0	0.2	0.0
NELL1	Neural EGFL-like 1	0.0	0.3	0.0
SLC17A7	Solute carrier family 17 member 7	0.0	0.6	0.0
CHATb	Choline acetyltransferase	0.0	0.0	0.0
OLIG2b	Oligodendrocyte transcription factor	0.0	0.0	0.0

^aShown are expression levels (transcripts per million reads [TPM]) of 26 highly neuron-specific marker genes selected from the Allen Brain Atlas (82–84; http://brain-map.org) and BioGPS (85, 86; http://biogps.org) as examined by RNA sequencing.

^bGenes selectively expressed in motor neurons were selected from https://en.wikipedia.org/wiki/Neuronal_lineage_marker.