Figure 2.

Amino acid sequences of a portion of CCR5. (A) Comparison of the human and owl monkey CCR5s with differences highlighted in grey and yellow. Sulfated tyrosines positions 10 and 14 shown to be important for gp120-CCR5 interaction are highlighted in blue. Figure adapted from Huang et al. 2007. Schematic representation of human CCR5. Sequence corresponds to the extracellular N-terminus of CCR5 (17). Residues of interest are depicted in red. Extracellular loops and disulfide linkages (-SS-) are labeled. (B) Effect of amino acids Y15 and T16 on the ability of owl monkey CCR5 to function with human CD4 as a coreceptor for different HIV-1 variants. 293T cells stably expressing the permissive human CD4 receptor and indicated CCR5 coreceptor were infected with pseudovirus with the Envs indicated on the x-axis. Infection was measured by flow cytometry as percent GFP-positive cells 48hrs postinfection. Error bars represent the standard deviations between replicate wells from 2 separate experiments.