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. Author manuscript; available in PMC: 2018 Oct 17.
Published in final edited form as: Science. 2018 Jul 5;361(6402):604–607. doi: 10.1126/science.aat5671

Fig. 2. Synapsin 1 drives phase separation of SH3 domain containing binding partners.

Fig. 2.

(A) and (B) Full-length synapsin 1 (10 μM) and either Grb2 (10 μM), or the SH3 domain containing region of intersectin (10 μM) form droplets under physiological conditions. Top: domain organization of the proteins. Bottom: fluorescence images of the protein mixtures at 30 and 45min. (C) and (D) Fluorescence images of the solution immediately (within one min) after mixing of the two proteins in the presence of the crowding reagent (3 % PEG 8,000). (E) and (F) Fluorescence recovery of synapsin 1 after photobleaching a region within a synapsin 1-Grb2 droplet, or a synapsin 1-(SH3)5-intersectin droplet (see insets). Error bars represent s.e.m., red is the fit with a hyperbolic function.