Fig. 3.

NS6740 selectively modulated α7 nAChR ligand-gated channels. A) Control applications of 60 ACh were delivered to oo-cytes expressing α7 (n = 8, second of two ACh controls shown). Subsequently, 5 μM NS6740 was applied for 60 s prior to another application of ACh. This was followed by two additional control applications of ACh at four- minute intervals. The data shown are the point-by-point averages, solid line) of the data from each cell after they were normalized to the amplitude of the initial ACh responses from each cell. The standard errors of the mean at each point are plotted as the shaded area. B) Responses of cells expressing α7 prior to and following the application of 5 μM NS6740 were compared to those of other receptors important for hippocampal physiology: α2β2 nAChR, AMPA-type glutamate receptors (cells co-expressing GluRl and GluR2), and a representative GABAa receptor (cells co-expressing al, β2, and γlL subunits) using the same application protocol illustrated in A. The α7 responses were calculated as net charge and the others as peak currents. ACh control concentrations used were 10 μM and 100 μM, respectively for the high sensitivity and low sensitivity forms of the α2β2 receptors (see Methods), 100 μM kainic acid was the control for the glutamate receptors, and 10 μM GABA