Fig. 4.

A) Responses of oocytes expressing α7 (n = 8) showing the effect of 100nM MLA on α7 receptor response to 60 μM ACh application. After the acquisition of control responses to ACh (the second of two controls is shown), MLA was applied for 60s prior to a second application of ACh. This was followed by two additional control applications of ACh at four- minute intervals. B) Responses of oocytes expressing α7 (n = 8). After the acquisition of control responses to ACh, nM MLA was applied for 60 s prior to a second application of ACh. This was followed by an application of 10 μM PNU-12096, a PAM of α7 nAChR. C) Responses of oocytes expressing a7 (n = 8) showing that the effect of NS6740 on the α7 receptors was distinct from simple inhibition produced by MLA, as shown by the ability of the PAM PNU-120596 to evoke large responses from the receptors that were unresponsive to ACh. The data in each trace are the point-by-point averages (solid line) of the data from each cell after they were normalized to the amplitude of the initial ACh responses from each cell. The standard errors of the mean at each point are plotted as the shaded area.